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Introduction to glycolysis . Role of glycolysis in producing ATPs and NADHs and converting glucose to pyruvates. How pyruvate from glycolysis is converted to acetyl CoA so it can enter the citric acid cycle . Pyruvate is modified by removal of a carboxyl group followed by oxidation, and then attached to Coenzyme A. The Krebs cycle occurs in the mitochondrial matrix, where pyruvate is turned into acetyl-CoA, which then goes through a series of reactions, producing ATP, NADH, and FADH2. Glycolysis , the citric acid cycle , and the electron transport chain work together to release chemical energy from glucose and store it in ATP. Glycolysis uses glucose to produce ATP and NADH.