Thus, glycolysis produces both ATP for cellular energy requirements and building blocks for synthesis of other cellular products.This process occurs in all organisms, and is responsible for converting glucose to pyruvate and generating ATP in the process. The process by which the glucose (6C compound) is split into two molecules of pyruvic acid (3C compound) is called glycolysis or EMP pathway. The glucose is broken down anaerobically to form pyruvic acid. The pyruvic acid enters the Krebs cycle. Glycolysis occurs in the cytoplasm of the cell. The pyruvic acid is the end product of this process. Uses: 1) Glycolysis is the first step used for the breakdown of glucose for extraction of energy. In anaerobes, it is the only method for extraction of energy. 2) The process overall produces two pyruvate molecules, two net ATP molecules, and two NADH molecules for the cell to use them as energy currency. Was this answer helpful? Glycolysis is the first stage of the breakdown of glucose in the cell. During glycolysis 2 ATP molecules are used up and four ATP molecules are generated. In the entire process of glycolysis , two NADH₂ molecules are also generated. When these molecules undergo ETS they will form 3 ATP per NADH₂ which means 6 ATP.