Coombs test: 1、简述血凝实验的原理、意义和结果的判定方法

1、简述血凝实验的原理、意义和结果的判定方法。血凝试验又称为Coombs试验，是检测抗红细胞不完全抗体的一种的方法。还可采用专一特异性的抗球蛋白的血清如IgG血清、抗IgA或抗IgM以及抗补体血清等，分析结合于红细胞 Coombs test / DAT is used to determine whether a patient’s red blood cells (RBCs) are coated with immunoglobulins or complement in vivo The pediatric hematologist is concerned about autoimmune hemolytic anemia and orders a direct antiglobulin test (DAT), which is negative and requests a peripheral blood smear, which shows increased spherocytes and polychromasia in the image above. Positive Coombs test , evidence of extravascular rather than intravascular hemolytic anemia (Oxf Med Case Reports 2020;2020:omz125) Various presentations of microangiopathic and macroangiopathic anemia Sickle cell disease: Vaso-occlusion and vasculopathy with early onset and chronic progression (Intern Emerg Med 2019;14:1051)