Biuret test: Is used for the

Biuret test is used for the detection of peptide bonds in the protein. This test is not suitable for amino acid only. The protein solution when reacts with CuSO4 in alkali condition (NaOH/KOH), purple to violet-colored copper (II) cation complex is formed. The Biuret Test is a classic biochemical assay widely utilized to identify the presence of proteins in a given sample. This colorimetric test is based on a reaction between peptide bonds and copper ions, resulting in a distinctive color change. With its simplicity and effectiveness, the Biuret test for protein is a crucial method in biological, food, and chemical analysis laboratories. Principle of the Biuret Test The Biuret Test operates on the fundamental principle that peptide bonds in ... Biuret reagent is essential in biochemical analysis, serving a key role in both qualitative and quantitative protein detection. This comprehensive guide explores its composition, preparation, underlying principles, applications, and significance of the Biuret test, a vital technique used in laboratories globally. Biuret Test The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can be also be used to gauge the amount of protein present in the analyte. In this test, the presence of peptides results in the formation of pale purple coloured coordination compounds of the copper (II) ion (when the solution is sufficiently alkaline). It can also be noted that the intensity of the purple colour and, therefore, the ...