Tissue culture is defined as a technique used to grow cells, tissues, or organs in a controlled, sterile environment. It allows scientists to produce thousands of identical plants from a single parent plant, making it a powerful tool in plant propagation. This method is especially useful in agriculture as part of strategies for enhancement in food production, helping in the rapid multiplication of high-yielding and disease-resistant crops. Tissue culture, a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. Plant tissue culture is a technique plant breeders use to grow and reproduce new plants from pre-existing ones. It involves cultivating plant cells, tissues, or organs in an artificial medium under controlled temperature, light, and humidity. In tissue culture , cells or tissues are removed from an organism and grown in the laboratory using a nutrient medium that provides the nutrients and growth factors the cells need to grow.