Gram stain is a technique to classify bacteria into gram -positive and gram -negative based on their cell wall properties. Learn how it works, who invented it and when it is used in medicine and research. Gram staining is a differential bacterial staining method used to distinguish between Gram Positive and Gram Negative bacteria based on the makeup of their cell walls. There are many different types of staining the most important one is gram staining . It is both the most used and significant staining method in bacteriology, particularly in medical bacteriology. It is frequently the initial test carried out on bacteria during the process of their identification and observation. History: Hans ... Learn how to differentiate gram positive and gram negative bacteria using crystal violet, iodine, safranin and alcohol. See the animation, examples and reagents used in gram staining technique. Reagents: Primary Stain - Crystal Violet Mordant - Grams Iodine Decolourizer - Ethyl Alcohol Secondary Stain - Safranin Introduction- Gram staining is a fundamental microbiological technique used to differentiate bacterial cells into two major groups based on their cell wall composition. Developed by the Danish physician Hans Christian Gram in 1884, this technique has since become a cornerstone in bacterial identification and classification due to its simplicity and effectiveness. Principle ...
